Fig. 1.
Evaluation of periostin expression in Sgcd−/− mice. (A) Immunohistochemistry showing periostin (green) localization in muscle biopsy specimens from normal patients and patients with Duchenne MD (DMD). (Original magnification: 600×; scale bar: 50 μm.) (B) Periostin levels, detected by ELISA, in the serum of 6-wk old WT (Wt) and Sgcd−/− mice. *P < 0.05. n = 4 mice each. (C) Western blot for periostin in Wt and Sgcd−/− mice from the diaphragm at 6 wk and 6 mo of age. GAPDH was used as a loading control. (D) Immunohistochemistry showing increased periostin (green) in areas of fibrosis in the diaphragm (diaph.), gastrocnemius (gastroc.), and quadriceps (quad.) of Wt and Sgcd−/− mice at 6 wk and 6 mo of age. Membranes are stained in red with wheat germ agglutinin conjugated to TRITC, and nuclei are stained with DAPI (blue). (Original magnification: 400×; scale bar: 100 μm.) (E) Immunohistochemistry showing ZsGreen staining (green) of the gastrocnemius in Sgcd−/− and Sgcd−/−Postn-ZsGreen TG mice. The ZsGreen-positive cells express periostin. Wheat germ agglutinin was conjugated to TRITC (red) and To-Pro nuclei (blue). Error bars represent SEM.