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Increased amounts of stroma in tumors grown in Cav-1 KO fat pads. A complex collagen network was detected in H&E-stained tumors by an intense pink staining. Masson's trichrome stain also reveals the presence of collagen, as depicted by a blue stain. To detect the presence of fibroblasts embedded within the collagen network, tumors were immunostained with a vimentin antibody. Cav-1 KO tumors have more vimentin-positive cells. Arrows, stromal cells. Original magnification, ×60.
