Fig. P1.
General workflow for the analysis of live colonies by nanoDESI, followed by global mass spectral data visualization by using Cytoscape, allowing for more efficient data mining and compound identification. First, the colonies grown on agar surfaces are subjected to atmospheric MS analysis. Each molecule detected by MS is then fragmented, and a similarity matrix is applied to find related spectra. Related spectra are then visualized as a network, which, in turn, facilitated compound identification.