Fig. 3.
Endogenous IFI16 is acetylated within the NLS and mediates a type I IFN response in lymphocytes. shRNA-mediated knockdown of IFI16 (A) compromises IFN-β expression in response to VACV 70mer transfection in CEM-T lymphocytes (B). (C) Combinatorial mass spectrometric approach to identify PTMs on endogenous IFI16 from CEM-T cells. (D) Coomassie-stained SDS/PAGE shows efficient IFI16 isolation; dotted lines indicate IFI16 isoforms. (E) Map of IFI16 phosphorylations (red pins) and acetylations (green pins). (F) Identification of NLS acetylations (ac) using ETD and CID MS/MS.