Figure 2.
Acute allergen challenge leads to an increase of EPCs in the lungs. (A) Bars represent mean ± SEM of the numbers of EPCs at 21 days of culture, as described in Materials and Methods. * represents p < .05 compared to alum controls. Photomicrograph represents lung EPC colonies in bright-field; magnification ×50 (inset: ×400). (B) Representative photomicrographs from EPCs immunostained for CD31-Alexa 488/DAPI; vWF-Alexa 564/DAPI, and VEGFR2/Alexa 564/DAPI. (C) Representative photomicrographs from EPC colonies positive for Ac-LDL uptake and GS-lectin. (D) Representative photomicrographs from hematopoietic colonies (left and middle) or EPCs (right) immunostained for CD45- and CD14-positive monocytes. Data derived from 8–12 mice. Abbreviations: Ac-LDL, acetylated low-density lipoprotein; DAPI, 4′,6-diamidino-2-phenylindole; EPC, endothelial progenitor cell; GS-lectin, Griffonia simplicifolia isolectin; vWF, von Willebrand factor.