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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Nat Cell Biol. 2012 Mar 4;14(4):401–408. doi: 10.1038/ncb2464

Figure 2. Loss of Lrig1 causes crypt expansion.

Figure 2

a-b) Grossly enlarged abdomen in postnatal day 10 Lrig1 KO animals. c-d) Lrig1 KO animals are smaller than control littermates but the length of the gut is not affected. Length of the gut and weight of neonates collected from P4 to P10. (P4:WT/Het n=7, KO n=3; P6:WT/Het n=15; KO:n=7; P8:WT/Het n=9, KO n=5; P10:WT/Het n=16, KO n=7). Error bars represent s.d. and asterisks significant changes (P6: p=0.0028; P8: p=0.011; P10: p=5.5×10−5). e-f) Loss of Lrig1 causes crypt expansion. H&E staining of proximal jejunum from Lrig1 WT and KO littermates. White lines demarcate crypt structures. g-h) The crypts from Lrig1 KO animals contain more proliferating cells. Phosphorylated histone H3(Ser10) (green) in intestinal samples from Lrig1 WT and KO littermates counterstained with dapi (blue). Scale bars 100μm (e-f), 50μm (f-g).