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Repetitive stimulation. Neuron firing frequency rates are shown in response to optical stimulation with pulse frequencies ranging from 1 to 200 Hz. Top: response at different stimulation irradiance levels: 100%, 120%, and 140% of single pulse action potential threshold (Ithres). Bottom: response with different ChR2 channel densities: 75%, 100%, and 150% of our default density (ρChR2). Irradiance level was 120% of single spike threshold.
