Figure 3.
Lyn is hyperphosphorylated in Tg6/λ-MYC tumors. (A) Representative western blots of cervical tumors for phosphorylated Lyn (pLyn), total Lyn (tLyn), LMP2A, and GAPDH from 4 Tg6/λ-MYC (6M) and 4 λ-MYC (myc) transgenic mice (a non-specific band consistently detected with anti-LMP2A antibody is also seen just above the LMP2A). (B) Densitometric analysis of pLyn normalized to tLyn for each tumor sample. The raw densitometric value for each pLyn band from each sample is divided to that of tLyn from the same sample and multiplied by 100 to find the percent of pLyn for the respective mouse. Data are from 4 independent experiments, totaling 14 Tg6/λ-MYC and 15 λ-MYC transgenic mice. See Material and Methods for the details of data presentation on the graphs.
