FIGURE 7.
PAR-4 and ceramide are associated with exosomes secreted by amyloid peptide-treated astrocytes. A, control cells not treated with amyloid do not show co-distribution of PAR-4, ceramide, and TSG101. Bar = 2 μm. B, Aβ25–35 treatment induces co-distribution of PAR-4, ceramide, and TSG101, which is predominantly found in the perinuclear region and bleb-like membrane structures on the surface of astrocytic processes (arrow). Bar = 1 μm. C–E, incubation of astrocytes with Vybrant CM-DiI-labeled exosomes shows co-distribution with TSG101, ceramide, and PAR-4 (profile analysis of pixel intensity in E). Bar = 3 μm.