FIGURE 6.
PICALM manipulation via viral gene transfer alters Aβ synthesis in hippocampus of APP/PS1 mice. Dissected hippocampi from PICALM knockdown and overexpression animals were homogenized first in PBS and then in 5 mm guanidine, and Aβ was quantified using ELISA. PICALM knockdown resulted in an ∼30% decrease in PBS-soluble Aβ40 and Aβ42 levels (A). In addition, insoluble guanidine fractions showed a similar decrease in Aβ40 and Aβ42 levels (B). Conversely, PICALM overexpression resulted in a 40–50% increase in PBS-soluble Aβ40 and Aβ42 levels (C) and a 20–40% increase in insoluble Aβ40 and Aβ42 (D). Values are mean ± S.E. (error bars); n = 7–9 mice/group. *, p < 0.05. shScr, shScrambled.