FIGURE 1.
APP co-localizes with PICALM after endocytosis. Live N2a-APP695 cells were surface-labeled with 6E10 antibodies at 4 °C to prevent endocytosis. The cells were then warmed to 37 °C for varying times to allow internalization, fixed, and immunostained for PICALM and surface-labeled APP. Prior to endocytosis (0 min), no co-localization of surface APP and PICALM was observed. Only after endocytosis was permitted was co-localization of surface-labeled APP and PICALM seen in intracellular vesicles.