Figure 6. Frequencies of different base substitutions within secondary recombinations.
(A) Scatterplot showing the incidence of different substitutions outside the primary locus relative to their frequency in secondary recombinations. Points are coloured according to the efficiency with which they are transferred through transformation when mismatch repair is active (see key). This shows that SNPs repaired effectively by mismatch repair are the most common, and are represented in secondary recombinations at the same level as those SNPs repaired less effectively. (B) Scatterplot showing the frequency of different substitutions when imported at the extreme ends of RSSs (x axis) relative to their frequency as the nearest flanking marker SNP, which is not converted to the donor allele (y axis); this compares the types of substitution found at the two polymorphic sites that define BRs. This fails to provide any evidence that localised mismatch repair processes may affect the positioning of transformation event boundaries or be the cause of the mosaic structure of recombinations, as SNPs repaired more efficiently by mismatch repair are approximately equally represented within, and surrounding, secondary RSSs.