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. 2012 Jun 14;8(6):e1002745. doi: 10.1371/journal.ppat.1002745

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Croucher et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Histogram showing the distribution of RSS sizes as L50_R lengths. The vertical green line shows the length of the cps locus in the recipient's genome (21,373 bp). The recombinations longer than this are the selected events importing the kanamycin resistance marker. The smaller, unselected recombinations are modelled as an exponential distribution (red line), with the calculated rate parameter displayed. (B) Histogram showing the distribution of donor molecule lengths participating in primary recombinations, as estimated using the L50_D lengths of the NCR boundaries. The vertical green line shows the length of the kanamycin resistance locus in the donor DNA (1,354 bp). (C) Histogram showing the distribution of secondary RSS sizes as L50_R lengths. These are modelled as an exponential distribution, indicated by the red line and displayed rate parameter. (D) Histogram showing the distribution of secondary NCR L50_D lengths, thereby estimating the sizes of donor molecules participating in secondary recombinations. These are also modelled as an exponential distribution, indicated by the red line and annotated rate parameter.