Figure 5. Fluidigm analysis of CD8+ T-cells specific for HLA-A2 NLV epitope of CMV.
PBMC were stimulated with NLV peptide for three hours (without monensin or brefeldin A). Results are presented as a heat map, where each row represents the gene expression profile of a single cell (e.g., cell A and cell B, pink box), and each column represents the expression level of one of the assayed genes (e.g., transcript I and transcript T, pink arrows). Levels of each transcript are potted on a gray scale, from low expression (white) to high expression (black). To simplify presentation, individual gene names are not shown; instead, genes are grouped into modules, as indicated at the bottom of the figure. The final four columns in the map depict composite expression profiles, which show the total level of expression for all the measured transcripts (1), and for those transcripts associated with cellular activation (2), cytolysis (3), and cytokines (4), within each cell.