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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Methods. 2012 Feb 25;57(3):251–258. doi: 10.1016/j.ymeth.2012.02.009

Figure 4. Properties and applications of BV421.

Figure 4

A) Schematic illustrating polymer structure of BV421, as conjugated to antibody. Energy from a violet laser (hva) excites electrons in the polymer and blue light (hve) is emitted. Excitation (purple) and emission spectra (blue) for BV421 are also shown. B) Staining patterns for BV421 (left), Pacific Blue (read on the same channel as BV421, middle), and PE (right) conjugates of anti-human CD8. C) Identification of CMV-NLV specific CD8+ T- cells using pMHCImultimers made with streptavidin-BV421 (left), -APC (middle), or –QD605 (right).