Figure 4.
Enhanced metastatic potential of human colon cancer cells in K14-hIL-8/nu mice (A) Bright field, red fluorescent and combined images of cultured HCT116LR cells ( × 100) (B) RFP signals from both WT nude and K14-hIL-8/nu mouse. Both mice were skinned to better detect the RFP signal. (C) Liver, lung, heart, kidney, spleen and subcutaneous lesions were harvested from K14-hIL-8/nu mice and their bioluminescent signals from firefly luciferase and fluorescent signals from RFP were simultaneously captured. (D) Bright field and red fluorescent images showing lung metastases in the WT nude and K14-hIL-8/nu mice. (E) Average number of metastatic lesions in the lung, liver and other organs (skin, heart, spleen and kidney) in the WT nude (n=5) and K14-hIL-8/nu (n=6) mice. Graph represents the average number of total metastases in the WT nude vs K14-hIL-8/nu mice. *P<0.05. The colour reproduction of this figure is available at the British Journal of Cancer online.