TABLE 1.
Primer sequences for validation of novel transcripts identified by RNA-Seq
| Gene | Method | Amplification | Direction | Sequence (5′ to 3′) | PCR Product |
|---|---|---|---|---|---|
| bp | |||||
| Cyp2b10 | Endpoint PCR | From exon 6 to exon 9 (including cassette exon) | Forward | ATGGCTTCCTGCTCATGCTCAAGT | 411 |
| Reverse | GACAAATGCGCTTTCCCACAGACT | ||||
| Sequencing | From exon 7 to exon 9 | AGTGCCACACAGAGTGACCAAAGA | |||
| Cyp2d26 | Endpoint PCR | From exon 5 to cassette exon | Forward | ACTACACATCCCTGGTTTGCCTGA | 520 |
| Reverse | AGCCTCTGAGCACCTTCTCTTGTA | ||||
| Sequencing | From exon 6 to cassette exon | TGATTGACCTGTTCATGGCAGGGA | |||
| Endpoint PCR | From cassette exon to exon 9 | Forward | TACAAGAGAAGGTGCTCAGAGGCT | 327 | |
| Reverse | TAGGGCTCTGGAGTAACTGGCATT | ||||
| Sequencing | From exon 8 to cassette exon | CATGAAGGCCTCGTGCTTCACAAA | |||
| Cyp3a13 | 3′-RACE | cDNA synthesis | GCGAGCACAGAATTAATACGACTCACTATAGGT12VN | ||
| Outer PCR | Forward | AAGTTGCTCTTGTCAGAGTCCTGC | |||
| Reverse | GCGAGCACAGAATTAATACGACT | ||||
| Inner PCR | Forward | CACTGTCCAGCCTTGTAAGGAAAC | |||
| Reverse | CGCGGATCCGAATTAATACGACTCACTATAGG | ||||
| Sequencing | AAGTTGCTCTTGTCAGAGTCCTGC |