Figure 2.
Syndecan-1 expression is associated with apoptosis resistance and enhanced cell proliferation. (a) Cell proliferation after 48 h of incubation in decreasing serum concentrations: RPMI 10% FBS (black bars), 5% FBS (grey bars) and 2% FBS (light grey bars), and, as control, culture media (complete EBM 10% FBS, white bar). Data are expressed as the mean±s.d. of four experiments performed in triplicate. Analysis of variance with Dunnett multiple comparison test was performed (*P<0.01 MMECs versus HUVECs; †P<0.05 MMEC syndecan-1 siRNA versus MMECs). (b) Apoptosis after 24 h of incubation in the presence (black bars) and absence of serum (grey bars), and, as control, in culture media (complete EBM 10% FBS, white bars). Data are expressed as the mean±s.d. of four experiments performed in triplicate. Analysis of variance with Dunnett multiple comparison test was performed (*P<0.001 MMECs versus HUVECs; †P<0.001 MMEC syndecan-1 siRNA versus MMECs).