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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Cancer Cell. 2012 Mar 20;21(3):374–387. doi: 10.1016/j.ccr.2011.12.028

Figure 4. Knock-down of Gli1 in EAC cells decreases cell proliferation, migration, invasion, and colony formation.

Figure 4

A) The BE3 stable clones with Gli1 knock-down. C: non-silencing control shRNA; #1-5: 5 different Gli1 shRNAs.

B) The mRNA levels of Gli1 target genes in the BE3 stable clones with Gli1 knock-down measured via real-time PCR and normalized to the mRNA level of ACTIN. Error bars represent SD (n = 3).

C) Cell counting analysis of the BE3 stable clones with Gli1 knock-down. All cells were counted, and then 1 x 105 cells were seeded in 10-cm dishes. Error bars represent SD (n = 3).

D) MTT assay of the BE3 stable clones with Gli1 knock-down. All cells were counted, and then 3,000 cells were seeded in each well of a 96-well plate. Error bars represent SD (n = 3).

See also Figure S4.