Fig 5.
Replication of sfRNA mutant WNVKUN is partially rescued in RNase L−/− but not in PKR−/− MEFs. (A) Northern blot of viral RNA in wt and PKR−/− MEFs infected with wt (FLSDX) and sfRNA-deficient (FL-IRAΔCS3) viruses. (B) Corresponding titers of infectious virus in the supernatant of the infected cells. The data are the average of two independent experiments. Error bars indicate standard deviations. (C) Northern blot of viral RNA in wt MEFs or RNase L−/− MEFs infected with wt (FLSDX) and sfRNA-deficient (FL-IRAΔCS3) viruses. (D) Corresponding titers of infectious virus in the supernatant of the infected cells. The data are the average of two independent experiments performed in duplicate. Error bars indicate standard deviations. Viral RNA was detected in all Northern blots with a 3′ UTR-specific probe. Ethidium bromide staining of rRNA is included as a loading control for each blot.