Figure 4.
Crystallin expression is variable in individual brains from flu-exposed embryos. Expression of crystallin αA (cryaa), crystallin βA1 (cryba1) and crybb1 was assessed by quantitative PCR (qPCR) across the individual brains of 36 flu and 36 saline embryos. Each symbol represents a phosphoglycerate kinase 1 (PGK1)-normalized gene expression level in a single embryonic brain. The dashed gray line denotes the reliable transcript detection threshold by qPCR. Note the upregulation of the three crystallin genes in the flu group compared with controls, and the considerable variability of crystallin expression levels between the flu-exposed embryonic brains.