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. 2012 Apr 3;2(4):e98. doi: 10.1038/tp.2012.24

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Copyright © 2012 Macmillan Publishers Limited

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Quantitative PCR (qPCR) validates crystallin gene expression changes across the maternal immune activation (MIA) models. The expression of nine genes was tested (crystallin αA (cryaa), crystallin γB/C/A (crygb/c/a), crystallin γC/A (crygc/a), crystallin βB1 (crybb1), crystallin βA2 (cryba2), crystallin βB3 (crybb3), crystallin βA1 (cryba1), crystallin βA4 (cryba4), crystallin αB (cryab)) on pools of RNA derived from the three MIA treatments. The X axis denotes average log 2 ratios reported by microarrays, whereas the ddCt qPCR values are plotted on the Y axis. Note that the qPCR and microarray-reported expression changes are highly correlated across the three MIA models.