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. Author manuscript; available in PMC: 2012 Oct 13.
Published in final edited form as: Mol Cell. 2012 Mar 8;46(1):67–78. doi: 10.1016/j.molcel.2012.02.005

Figure 5. Reduced Stability of Fbw7 in Glmn Depleted Cells Contributes to Cyclin E and c-Myc Accumulation.

Figure 5

(A) Lysates from E9.5 Glmn+/+, Glmn+/− and Glmn−/− embryo and yolk sac were WB.

(B) Extracts from U-2OS cells transfected with Glmn, Fbw7 or control siRNA were WB. Fbw7 was immunoprecipitated with Fbw7 antibody followed by WB.

(C) Lysates from U-2OS cells transfected with Glmn or control siRNA and treated with CHX for the indicated time were WB. To normalize the amount of protein at time zero, blots highlighted in boxes were exposed for different times. Fbw7 was immunoprecipitated with Fbw7 antibody prior to WB.

(D) Extracts from U-2OS transfected with Glmn siRNA and EV, HA-Fbw7 or HA-Gsk3 plasmids were WB.

Sections of GVM were stained with H&E or immunostained with smooth muscle (SM) α-actin, Cyclin E (E), c-Myc (G), Rbx1 (I) or Cul1 (K) antibody. Squares in green outline fields enriched for glomus cells; squares in black shows field with normal VSMCs. Percentage of Cyclin E (F), c-Myc (H), Rbx1 (J), or Cul1 (L) positive cells present in sections of different GVM patients was quantified by Aperio analysis.

See also Figure S3