Figure 5. EtOH induced changes in the buoyancy of lipid-raft associated proteins.
Lipid-rafts were prepared by sucrose-gradient centrifugation from hippocampus of saline (Ctrl) and EtOH-treated wt (A) and PrP−/− (B) mice (5, 20 min post-EtOH i.p injection). An aliquot of each fraction was analysed by SDS-PAGE and blots were probed with the indicated antibodies. One representative of four independent experiments is shown in panels A and B. Amounts of immunoreactivity of PrP, p-Y1472GluN2B, p-Y416Src-PTK and flotilin-1 were determined in fractions 4 and 5 and expressed as the percent of total immunoreactivity measured in all fractions for each protein of interest. Results are representative of 2 mice/condition in 4 independent experiments and presented as mean ± SD (N = 4; Dunnett's post hoc; EtOH injections versus saline injections: *p<0.05; **p<0.01).