Table 3.
Functions of proteins O-GlcNAcylated in response to heat-stress
| kDa | Protein name | O-GlcNAcylated in response to stress | Complexes with O-GlcNAcylated proteins | Function | Stress-associated functions |
|---|---|---|---|---|---|
| 60 | NUP54 | – | ? | Nuclear transport | O-GlcNAcylation in response to oxidative stress is associated with inhibition of nuclear import and export (Crampton et al. 2009; Kodiha et al. 2009) |
| 240 | NUP214 | – | ✔ | Nuclear transport | O-GlcNAcylation in response to oxidative stress is associated with inhibition of nuclear import and export (Crampton et al. 2009; Kodiha et al. 2009) |
| 46 | CARM1 | ✔ | – | Methylates histone residues | CARM1 is involved in DNA damage-induced activation of GADD45 (Wysocka et al. 2006); co-activator of P53 (Scoumanne and Chen 2008); enhances tolerance to oxidative stress (Bauer et al. 2010); interacts with OGT (Cheung et al. 2008) |
| 150 | SEC31 | ? | ? | ER to Golgi Vesicle transport; binds Sec13 and p250—a phospholipase | ER stress reduces ER export (Amodio et al. 2009) |
| 150 | SEC24 B | ✔ | – | ER to Golgi Vesicle transport; binds Sec24 and p125i | Enhances survival under oxidative stress in Arabidopsis (Belles-Boix et al. 2000) |
| 190 | WNK1 | ✔ | – | Protein kinase | Involved in renal transport and hypertension (Wilson et al. 2001; Xu et al. 2005); regulation of TRPV4 (Fu et al. 2006), protects C. Elegans from hypertonic conditions (Choe and Strange 2007), regulated by hyperosmotic stress (Zagorska et al. 2007), regulates SPAK and OSR1(Vitari et al. 2005); Activates ERK5 (Xu et al. 2004); WNK3 regulates the interaction of caspase 3 activation (Verissimo et al. 2006) |
| 150 | NICE4 | ? | ? | ? | Phosphorylated upon DNA damage, probably by ATM or ATR |
| 140 | Zinc finger RNA-binding protein | ? | ? | Involved in postimplantation and gastrulation stages of development. Involved in the nucleocytoplasmic shuttling of STAU2—a protein that transports and targets mRNA | Enhances p53 transcript levels during stress |
| 140 | p125i | ✔ | – | ER to Golgi Vesicle transport; phospholipase binds sec23 | ER stress reduces ER export (Amodio et al. 2009) |
| 130 | SEC24-C | ✔ | – | ER to Golgi Vesicle transport; binds Sec24 and p125i | Enhances survival under oxidative stress in Arabidopsis (Belles-Boix et al. 2000) |
| 46 | NF45 | – | ✔ | Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Negatively regulates miroRNA processing | Negatively regulates miroRNA processing (Sakamoto et al. 2009), micro rna expression changes in response to stress (Leung et al. 2006; Marsit et al. 2006; Reyes et al.; Sunkar; Xu et al. 2003); binds PKR (Langland et al. 1999), binds Ku80 and Ku70 (Shi et al. 2007); interacts with DNA-PK (Ting et al. 1998) |
| 55 | TIP49α | ? | ✔ | Chromatin remodeling. ATP-binding proteins that belong to the AAA + (ATPase associated with diverse cellular activities) family of ATPases | DAN Damage repair & Apoptosis (Jha and Dutta 2009) |
| 190 | NUP153 | ✔ | – | Nuclear transport | O-GlcNAcylation in response to oxidative stress is associated with inhibition of nuclear import and export (Crampton et al. 2009; Kodiha et al. 2009) |
| 240 | Similar to gene trap ankyrin repeat | ? | ? | This gene encodes a protein with ankyrin repeats, which are associated with protein–protein interactions; also binds RNA | May be involved in host-virus interactions (Yeo and Chow 2007) |
| 55 | Tip49β | ? | ? | See Pontin (Above) | See Pontin (Above) |
| Proteins Identified as O-GlcNAcylated in response to stress, but not identified in the SILAC screen | |||||
| 90 & 110 | NF-90 | ✔ | ✔ | Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Negatively regulates miroRNA processing | Negatively regulates miroRNA processing (Sakamoto et al. 2009), micro rna expression changes in response to stress (Leung et al. 2006; Marsit et al. 2006; Reyes et al. 2010; Sunkar 2010; Xu et al. 2003); binds PKR (Langland et al. 1999), binds Ku80 and Ku70 (Shi et al. 2007); interacts with DNA-PK (Ting et al. 1998) |
| 460 | DNA -PK | ✔ | ✔ | Protein Kinase | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination |
√ Indicates that definitive evidence for this option was obtained in the study
? Indicates that suggestive evidence for this option was obtained in the study
- Indicates that no evidence for this option, or alternatives, was obtained in the study