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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Amino Acids. 2010 Jul 31;40(3):793–808. doi: 10.1007/s00726-010-0695-z

Fig. 4.

Fig. 4

Confirmation of proteins identified by the SILAC screen. Control, heat-stressed, or PUGNAc-treated cells were immunoprecipitated with either CTD110.6 or control IgM covalently coupled to cyanogen bromide activated Sepharose. Cell extract and immunoprecipitates were separated by SDS–PAGE and nuclear pore proteins (mAb414), NF-90, NF45, Carm1, Sec24, p125i, Tip49α, OGT, Actin, Tubulin, and HSP70 were detected by immunoblot