Fig. 3.
CDH17 knockdown in MHCC97H cells reduced their tumorigenicity in vivo. (A) MHCC97H cells transduced with CDH17 shRNAmir (shRNAmir) or nontargeted RNAi vector (Mock) and MHCC97H cells without treatment (97H) were transplanted into nude mice in a tumor xenograft model. Whole-body fluorescent imaging was performed to reveal the tumors (left panel). Once a week, four mice were killed and the subcutaneous tumors were removed. Tumor size and volume were photographed, and tumor volume was measured at each time point. A line chart illustrates the changes in volume of the subcutaneous tumors (right panel). (B) Effect of CDH17 shRNA suppression on the growth of subcutaneous tumors in nude mice. Subcutaneous tumors were induced in nude mice by injecting native (97H), scramble clone-transfected (Mock), or CDH17 shRNA-transfected (shRNA) MHCC97H cells (n = 3). Photographs illustrate the subcutaneous tumor produced by Mock and shRNA cells in nude mice. The volume of tumor induced in nude mice was measured among the three experimental groups after 35 days. *P = 0.0002.