Fig. 3.
Activated β-catenin increases the number of stem cells in the embryonic and neonatal cerebellum. (A-F) Cerebellar sections from E14.5 WT (A,C,E) or G-Cat (B,D,F) mice were stained with anti-GFAP antibodies (red in A,B) or anti-Sox1 (green) and anti-BrdU (red) antibodies (C-F). Note the increased expression of GFAP and Sox1 and the colocalization of Sox1 and BrdU in the mutant VZ. (G,H) Increased numbers of progenitors (G) and stem cells (H) in the cerebellum white matter in G-Cat mice compared with WT mice at P0. Cells isolated from the cerebellum of P0 WT and G-Cat mice were stained with antibodies specific for prominin 1 (Prom1) and for lineage (Lin) markers (PSA-NCAM, O4 and TAPA-1). The percentage of Prom1+ progenitors (G) and Prom1+ Lin– stem cells (H) was analyzed by flow cytometry. Data represent mean (± s.e.m.) percentages from three separate experiments. *, P<0.01. Scale bars: 50 μm.