Fig. 1.
SOAR dimer. (A) Schematic drawing of hSOAR. (B, C) Cartoon representation of the overall structure of the hSOAR monomer and the red-green hSOAR dimer. (D) Stereoview of the detailed interactions between the two monomers. The oxygen and nitrogen atoms are colored red and blue, respectively. Hydrogen bonds are shown by magenta dotted lines. (E) Western blots of coimmunoprecipitated wild-type and mutant hSOAR with the hOrai1 channel. (F) Localization of wild-type and mutant hSTIM1 transfected alone (first image in each row) and cotransfected with hOrai1. All hSTIM1 constructs were C-terminally tagged with EGFP and all hOrai1 constructs were N-terminally tagged with mCherry. Scale bars are 10 μm. (G) Fura-2 Ca2+ measurements of HeLa cells expressing hOrai1 alone or hOrai1 with wild-type or mutant hSTIM1. Inset, immunoblotting with anti-STIM1 antibody.