Figure 8.
Evaluation of hippocampal synaptic and axonal density in the brains of vehicle- or EpoD-treated 12-month-old mice PS19. A, B, Representative images (20×) of hippocampal CA3 synaptophysin immunostaining from 12-month-old vehicle-treated WT (A) and PS19 (B) mice, with the demarcated area quantified. C, Quantification of the CA3 synaptophysin-positive area in WT or PS19 mice treated with vehicle or EpoD at the indicated dose. D, E, Representative images (20×) of hippocampal NFL immunostaining from 12-month-old vehicle-treated WT (D) and PS19 (E) mice, with the demarcated area quantified. F, Quantification of the CA3 NFL-positive area in WT or PS19 mice treated with vehicle or EpoD at the indicated dose. All data are normalized to the mean area obtained in the vehicle-treated WT mice. Error bars indicate SEM; *p < 0.05, as determined by one-way ANOVA and Tukey's multiple-comparison test, with n = 8–10 mice/group.