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. Author manuscript; available in PMC: 2013 Mar 7.
Published in final edited form as: Cell Metab. 2012 Mar 7;15(3):372–381. doi: 10.1016/j.cmet.2012.01.018

Figure 6. Insulin signaling in aortic EC of VECKO mice.

Figure 6

(A-B) Representative immunoblots (A) and quantification (B) of insulin signaling in cultured aortic EC from VECKO following insulin treatment.

(C) Irs1, Irs2 and Igf1r expression in cultured aortic EC from VECKO and control mice. AU= arbitrary mRNA units.

(D) Illustration of conserved putative FoxO-binding sequences (red) in the Irs1 promoter and primers used for amplification of region A and B during ChIP. TSS: transcription start site.

(E) ChIP assay of cultured aortic EC using immunoprecipitation (IP) with rabbit IgG or antibody against FoxO1 and RNA polymerase (pol) II. Size of predicted PCR products sizes is 88 and 208 bp, respectively. The asterisk on the gel indicates a non-specific band. Data indicate means ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. controls.