Table 1.
Primers used for gene cloning
| Primersa | Sequences | Res. enzymesb |
|---|---|---|
| CODSEQ | 5′CCGAACATATGTCGTACTACCATCACCATCACCATCA- CGATTACGACATCCCAACGACCGAAAACCTGTATTTTC- AGGGCGCCATGGCTGATATCGGATCCGGAA3′ |
NdeI/Ba mHI |
|
| ||
| HISTEV-F | 5′CCGAACATATGTCGTACTAC 3′ | NdeI |
| HISTEV-R | 5′TTCCGGATCCGATATCAGCC 3′ | BamHI |
| HISGFP-F | 5′GCGACATATGTCGTACTACCATCACCATCACCATCAC GATTACGATATGGTGAGCAAGGGCGAGG 3′ |
NdeI |
| HISGFP-R | 5′TCAGCCATGGCGCCCTGAAAATACAGGTTTTCGGTCG TTGGGATATCGTAATCCTTGTACAGCTCGTCC 3′ |
NcoI |
| NFIIIhd-F | 5′GGCGCCATGGAGGGCTTGAGCCGTAGGAGG3′ | NcoI |
| NFIIIhd-R | 5′AATTCGGATCCTCATGGTGGGTTGATTCTTTTTTC3′ | BamHI |
| SP100-F | 5′CTGGTGTCATGACTTTTCGAAGC3′ | BspHI |
| SP100-R | 5′TTATTTTCGAATTCTTATGGCTG3′ | EcoRI |
| RanBP2-F | 5′GTGAAACCATGGAACCATTTGC3′ | NcoI |
| RanBP2-R | 5′GTTATGGATCCTTACTGAGATTTTTG3′ | BamHI |
| Mutasekp-F | 5′ATACCCATGGAAAGTAAAAAAATATTG3′ | NcoI |
| Mutasekp-R | 5′CCAAGGATCCTCATCGTACAGAAAC3′ | BamHI |
| pTP-F | 5′CATGGTCATGAACCTTGAGCGTCAAC3′ | BspHI |
| pTP-R | 5′ATGCGAATTCCTAAAAGCGGTGACGC3′ | EcoRI |
| SSO2226-F | 5′GGCGCCATGGTAAAATATTCTATGCATTTAAAC3′ | NcoI |
| SSO2226-R | 5′AATTCGGATCCCTAAGGCCAACCTAAAATAG3′ | BamHI |
| TYLCVC2-F | 5′GGCGTCATGAGATCTTCGTCACCATCGACCAGCC3′ | BspHI |
| TYLCVC2-R | 5′AATTCGGATCCTTAAAGACTCTTAAAAAATGACC3′ | BamHI |
a
Forward primer (F) and reverse primer (R) used for PCR amplification.
b
Restriction enzymes used for cloning, the recognition sequence are underlined, BspHI-digested fragments were compatibly ligated into NcoI-digested vectors.