Figure 2. Inhibition of SCD1 activity induces growth arrest of CaP cells in vitro.
(A–D) Autoradiography of TLC showing quantification of SCD1 Δ9-desaturase activity on LNCaP (A), C4-2 (B) and PNT2 (C) cells by measuring the conversion of exogenous [14C]-saturated palmitic acid substrate into monounsaturated palmitoleic acid in control or 25 μM BZ36 treated cells. SCD1 desaturase activity was expressed as the ratio of palmitoleic acid to palmitic acid after normalization to cellular DNA content (D). (E–G): Proliferation of tumoral LNCaP (E) and C4-2 (F) cells, and of benign PNT2 cells (G) were measured with a hematocytometer at 24 h, 48 h and 72 h following culture with increasing doses of BZ36 (H) SCD1 immunoblot in LNCaP and C4-2 cells cell lines 24 h and 48 h after transfection with control or siRNA against hSCD1. (I–J) Proliferation of LNCaP (I) and C4-2 (J) cells was measured by counting the % of BrDU incorporation at 24 h and 48 h after siRNA transfection. Values are expressed as mean +/− sd and are representative of at least two independent experiments performed in triplicates.