Fig. 6. Th2 variant αGC acC8 fails to induce IL12 in vivo.
B6 mice were injected intraperitoneally with 2μg αGC or 5μg αGC acC8. (A) Splenocytes were harvested 6 h later and intracellularly stained for IL-12p40. Top row shows representative FACS plots of gated CD11c+ DC cells co-stained with anti-CD8α. Central row shows gated CD11blo CD11clo cells costained with anti-F4/80. Bottom row shows cells in the lymphoid gate costained for B220. Data representative of 4 experiments with 2 to 3 mice per group. (B) Mice were injected as in A with 2μg αGC or 5μg αGC acC8 and compared with mice injected with the mixture of 2μg αGC and 5μg αGC acC8. Top row shows DC staining for IL-12p40 as in A; bottom row shows staining of TCRβ− B220− splenic cells for DX5+ and intracellular IFN-γ. (C) Serum IL12-p70 at 6 h, IL-4 at 2 h and IFN-γ at 24 h post injection of 2μg αGC, 5μg αGC acC8 or the mixture as indicated.