Fig. 5. Antigen presenting cell requirement for αGC acC8 and other variants in vivo.
Δ/Δ mice and their littermate controls were injected with 5 μg αGC acC8 intraperitoneally. (A) Serum IL-4 at 2 h in cd11c Δ/Δ, lyz2 Δ/Δ, cd19 Δ/Δ and littermate controls. Serum IFN-γ at 24 h was undetectable. Data are normalized to the mean of littermate controls. (B) WT and Δ/Δ splenic NKT cells gated as TCRβ+ CD1d-αGalCer+ were stained for intracellular IL-4 and IFN-γ 2 h post injection. Left, representative FACS plots. Representative of 2 independent experiments with 2 to 3 mice per group. (C) Serum IFN-γ at 24 h and (D) serum IL-4 at 2 h post injection of 0.2 μg Th1 variant PBS57, 2 μg Th1 variant C-glycoside or 5 μg Th2 variant αGC acC20:2. IL-4 was indetectable for C-glycoside and IFN-γ was indetectable for αGC acC20:2.