Figure 3. Sensitization to lapatinib.
A) ZR75 cells were transfected with siRNA’s, plated on laminin-5, and then treated with lapatinib for 3 days. B) BT474 cells expressing control or CD151-specific shRNA’s were seeded onto laminin-5 or non-coated plastic, and treated as in A. Note that increased efficiency of CD151 knockdown by siRNA (panel A), compared to shRNA (panel B) results in more dramatic lapatinib sensitization in panel A. Knockdown efficiencies are demonstrated in Supplemental Figs. S3A, S3B (for panel A) and in Supplemental Fig. S3C (for panel B).