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. Author manuscript; available in PMC: 2012 Mar 22.

Published in final edited form as: Pflugers Arch. 2011 Feb 23;461(4):461–468. doi: 10.1007/s00424-011-0937-2

Exemplar gating currents measured from cells expressing Ca_V3.3 channels (A) and ID12 channels (B). Initial phase of current activation before (solid line) and after (dashed line) equilibration with 30 μM Er³⁺ is enlarged in insets. (C) Q_on was evaluated by integrating the area under the gating current traces at the beginning of each depolarizing pulse. Values obtained from Ca_V3.3-expressing cells (n = 6) and ID12-expressing cells (n = 6) were averaged. Solid lines are fits of experimental data by the Boltzmann equation. Boltzmann fits normalized to unity are shown in the inset (Ca_V3.3 - solid line; ID12 - dashed line). (D) Q_off was evaluated by integrating the area under the gating current traces at the end of each depolarizing pulse and averaged. Solid lines are fits of experimental data by the Boltzmann equation. Boltzmann fits normalized to unity are shown in the inset (Ca_V3.3 - solid line; ID12 - dashed line). Voltage dependencies of Q_on ( - experimental points, solid lines – Boltzmann fits) from panels (C) and (D) are depicted together with voltage dependences of steady-state activation of ionic current in (E) for the Ca_V3.3 channel and in (F) for the ID12 channel.

Inline graphic — Exemplar gating currents measured from cells expressing Ca_V3.3 channels (A) and ID12 channels (B). Initial phase of current activation before (solid line) and after (dashed line) equilibration with 30 μM Er³⁺ is enlarged in insets. (C) Q_on was evaluated by integrating the area under the gating current traces at the beginning of each depolarizing pulse. Values obtained from Ca_V3.3-expressing cells (n = 6) and ID12-expressing cells (n = 6) were averaged. Solid lines are fits of experimental data by the Boltzmann equation. Boltzmann fits normalized to unity are shown in the inset (Ca_V3.3 - solid line; ID12 - dashed line). (D) Q_off was evaluated by integrating the area under the gating current traces at the end of each depolarizing pulse and averaged. Solid lines are fits of experimental data by the Boltzmann equation. Boltzmann fits normalized to unity are shown in the inset (Ca_V3.3 - solid line; ID12 - dashed line). Voltage dependencies of Q_on ( - experimental points, solid lines – Boltzmann fits) from panels (C) and (D) are depicted together with voltage dependences of steady-state activation of ionic current in (E) for the Ca_V3.3 channel and in (F) for the ID12 channel.