Figure 2.
Analysis of PER3 protein level in mouse hippocampus. (a) Per3 has significant expression variation in the BXDs. The wide expression range is shown by the PC1 of the Per3 probe sets (1421087_at, 1460662_at and 1421086_at). The extreme strains (red bars) are BXD2 and BXD22 with the lowest transcript level, and BXD28 and BXD42 with the highest transcript level. Strains are indicated along the x axis. (b) PER3 protein level in the hippocampus of the two high and two low strains was analyzed by western blot. PER3 protein bands are detected as doublets ranging from 131 to 150 kDa. (c) Densitometric analysis shows a significant expression difference between the strains that is consistent with transcript level. The difference was determined using Student's t test (*=P<0.01).