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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2012 Feb 15;188(6):2794–2804. doi: 10.4049/jimmunol.1102068

Figure 3. Processing of HLA-A*0301 and UL40 signal peptides.

Figure 3

(A) Alignment of the UL40 and HLA-A*0301 SPs illustrating the SPase cleavage sites plus the n, h (hydrophobic; underlined) and c sequences in HLA-A*0301 (B) In vitro translation of mRNA coding for the signal peptide plus 100 aa of UL40 (UL40/137; lane 1) in the presence of ER-derived microsomes (lanes 2-5) and (Z-LL)2-ketone (lane 3). One aliquot of microsomes was extracted with sodium carbonate and separated into pellet (Pel, lane 4) and supernatant (Sup, lane 5). Dots indicate the SPs; lane 6 shows in vitro-translated UL40 SP. (C) and (D) In vitro translation of mRNA coding for the signal peptide plus 100 aa of HLA-A*0301 (HLA-A/124; lane 1) or UL40 (UL40/137; lane 1) in the presence of selectively permeabilized 721.221 cells (lanes 2-3) and (Z-LL)2-ketone (lane 3). Dots indicate the SPs; lane 4 shows in vitro-translated reference SP.