Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Cancer Gene Ther. 2011 Nov 25;19(3):192–201. doi: 10.1038/cgt.2011.77

Figure 1. Construction and characterization of new virus vFasL.

Figure 1

(a) Schematic representation of the new vaccinia virus vFasL versus parental virus vJS6. Both are derivatives of WR strain virus with tk gene mutated. (b) Western blots analysis showing a ~40 kDa band in the vFasL-infected CV1 cells and not in the supernatants. (c) FasR (CD95) expression on B16 and MC38 cancer cells, and AML12 normal hepatocytes. Cells were stained with a PE-conjugated anti-mouse Fas antibody and then analyzed by flow cytometry. Green shading corresponds to cells stained the antibody for FasR and red shading corresponds to the isotype control.