Figure 5.
Suppression of Cyp1a2 and Cyp2e1 mRNA expression by ER stress. (A) Mouse primary hepatocytes were infected with adenovirus expressing GFP or XBP1s. RNAs were isolated 24 h after viral infection for RT-PCR analysis. Data are mean ± SEM from quadruplicate samples. (B) Mice were i.p. injected with tunicamycin (1.5 mg/kg). IRE1α-mediated XBP1 mRNA splicing and the level of XBP1s protein were measured by RT-PCR and Western blot, respectively. Data shown are from two independently treated mice. Each lane represents an individual mouse. (C) mRNA levels of indicated genes in the liver of tunicamycin-injected mice measured by qRT-PCR. n = 2 mice per group.