Figure 4.
Molecular interactions of ERECTA family RKs and TMM in vivo. Arabidopsis wild-type (wt) or null mutant seedlings expressing epitope-tagged receptors are indicated in each panel. In each panel, both inputs and co-IP fractions (IP α-GFP) were subjected to Western blot (WB) with anti-GFP (α-GFP) or anti-Flag (α-Flag) antibodies. (A) ERECTA associates with itself. (B) ERL1 associates with itself. (C) TMM does not associate with itself in vivo. Reciprocal co-IP assays resulted in no TMM bands in the co-IP fractions. (D) ERECTA associates with TMM. (E) ERL1 also associates with TMM. (F) ERECTA associates with ERL1, suggesting heteromerization among ERECTA family RKs. Immunoblots of inputs of TMM-HA and TMM-YFP yielded nonspecific bands. Co-IP experiments were performed at least three times.