Table 1. Strains and plasmids.
| Strain # | Description | Reference |
|---|---|---|
| RP1831 | PAO1F (wild-type PAO1) | (62) |
| RP1871 | PAO1F ΔpscD | this study |
| RP1883 | PAO1F ΔexoS | this study |
| RP1945 | PAO1F ΔexoT | this study |
| RP1923 | PAO1F ΔexoY | this study |
| RP1947 | PAO1F ΔexoST | this study |
| RP1949 | PAO1F ΔexoS ΔexoT ΔexoY (Δ3TOX) | (35) |
| RP2750 | PAO1F ΔpopBD | this study |
| RP5479 | PAO1F exoS(GAP-) | this study |
| RP5481 | PAO1F exoS(ADPR-) | this study |
| RP5572 | PAO1F exoS(GAP-/ADPR-) | this study |
| RP6200 | PAO1F exoT(GAP-) | this study |
| RP6202 | PAO1F exoT(ADPR-) | this study |
| RP6206 | PAO1F exoT(GAP-/ADPR-) | this study |
| RP6203 | PAO1F exoS(GAP-) exoT(GAP-) | this study |
| RP6205 | PAO1F exoS(ADPR-) exoT(ADPR-) | this study |
| RP631 | PAK (wild type) | (55) |
| RP1600 | PAK ΔexoS | this study |
| RP1601 | PAK ΔexoT | this study |
| RP1603 | PAK ΔexoS ΔexoT | this study |
| RP1311 | PAK ΔpscJ | this study |
| Plasmid | Description | Reference |
|---|---|---|
| pPSV35 | Expression plasmid with lacIQ, lacUV5 promoter, gent resistance, colE1 and Pseudomonas origins of replication, oriT |
(63) |
| pP35-pscD | pscD cloned under control of lacUV5 promoter | this study |
| pEXG2-ΔpopBD | pEXG2 with popBD deletion allele (ΔpopB codon 3 to – popD codon 268) | this study |
| pEX-ΔexoS | pEXGW plasmid with exoS deletion allele | (30) |
| pEX-ΔexoT | pEXGW plasmid with exoT deletion allele | (30) |
| pEX-ΔexoY | pEXGW plasmid with exoY deletion allele | (30) |
| pEXG2-ΔpscD | pEXG2 with pscD deletion allele (Δ39-410) | this study |
| pEXG2-ΔpscJ | pEXG2 with pscJ deletion allele (Δ4-247) | this study |
| pEXG2-exoS(GAP-) | pEXG2 with exoS(R146K) mutant | (35) |
| pEXG2-exoS(ADPR-) | pEXG2 with exoS(E379D,E381D) mutant | (35) |
| pEXG2-exoS(GAP- /ADPR-) |
pEXG2 with exoS(R146K,E379D,E381D) mutant | (35) |
| pEXG2-exoT(GAP-) | pEXG2 with exoT(R149K) mutant | this study |
| pEXG2-exoT(ADPR-) | pEXG2 with exoT(E383D,E385D) mutant | this study |
| pEXG2-exoT(GAP- /ADPR-) |
pEXG2 with exoT(R149K,E383D,E385D) mutant | this study |