Figure 3. The role of popBD, ExoS, ExoT, and ExoY after infection with bacterial strains PAO1 or PAK.
A. Representative corneas, quantitative corneal opacity and CFU in C57BL/6 mice 48h after infection with 1 × 105 PAO1, ΔpscD or with mutants in the translocon apparatus (ΔpopBD); B. Corneas infected with PAO1, ΔexoS,T,Y (Δ3TOX) or ΔexoY mutants; C. Corneal infection with PAO1, Δ3TOX, ΔexoS, ΔexoT, or ΔexoST mutants; D. Corneas infected with strain PAK, type III secretion mutant ΔpscJ, or ΔexoS, ΔexoT, or ΔexoST PAK mutants. Data points represent individual corneas, which were analyzed by ANOVA, and p values for pair-wise comparisons from the post-hoc tests are shown. Overall ANOVA p value was <0.0001 for each set of studies. These experiments were repeated twice with similar results.
