Fig 5.
Genome-wide overlap between LXR and PPARα binding sites in mouse liver. (A) PPARα, LXR, and RXR ChIP-qPCR on selected loci in the livers of wild-type (WT) and PPARα knockout (KO) mice. Bars represent the mean plus range of two independent experiments. (B) Venn diagram representing the overlap between PPARα, LXR, and RXR binding sites in the livers of vehicle-treated WT mice. (C) Scatterplots illustrating the intensity of LXR and PPARα binding in vehicle-treated mouse liver (log2 number of reads per peak) at sites that are defined as LXR specific (blue), PPARα specific (red), and shared (green). The percentages indicate the fractions of sites that are above or below the broken line. (D) Venn diagram representing the overlap between PPARα binding sites in vehicle-treated WT liver with LXR and RXR binding sites in T0901317-treated (T09) WT liver. (E) Boxplot representing the number of PPARα ChIP-seq tags per peak in clusters that do not overlap with RXR and LXR binding (red), overlap with only RXR (orange) and overlap with both RXR and LXR (gray). Wilcoxon test with Bonferroni's correction was used. For all box plots, the rectangles show the IQR from the first quartile to the third quartile and the lines in the middle of the boxes represent the medians. The whiskers are drawn to the nearest value not exceeding 1.5 times the IQR, and outliers are not shown.