Fig. 3.
Effects of Aurora A knockdown on in vitro cell growth and apoptosis. (A) Aurora A knockdown in KCL-22, KCL-22M and K562 cells. Cells were analyzed 4 days after scrambled (mock) or Aurora A shRNA infection. (B) Aurora A knockdown induced apoptosis in KCL-22, KCL-22M and K562 cells. Cells were analyzed by annexin V staining 5 days after transduction. (C) Aurora A knockdown suppressed cell growth. Three days after mock or ShAuroraA infection, 1 × 105 cells were cultured in 1 ml media in triplicate in 24-well plate, and viable cells were counted at days indicated. (D) Aurora A knockdown inhibited clonogenic growth of CML cells. Three days after mock or ShAuroraA infection, 500 cells were cultured in standard two-layer soft agar. The numbers of colonies were counted with aid of microscope after 21 days.