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. Author manuscript; available in PMC: 2013 Jan 26.
Published in final edited form as: Neuron. 2012 Jan 26;73(2):317–332. doi: 10.1016/j.neuron.2011.10.038

Figure 4. Dopamine-induced Ca2+ release is independent of GHSR1a constitutive activity in cells co-expressing GHSR1a and DRD2.

Figure 4

(a) Co-expression of GHSR1a point mutants S123A and M213K that exhibit identical constitutive activity to WT-GHSR1a, and F297L which is devoid of constitutive activity Cells co-expressing DRD2 with either S123A or M213K fail to mobilize Ca2+ in response to dopamine, whereas the GHSR1a F279L point mutant exhibits Ca2+ mobilization in response to dopamine;

(b) Knockdown of Gαq subunit by siRNA does not inhibit dopamine induced Ca2+ mobilization in (left panel), whereas ghrelin-induced Ca2+ mobilization (right panel) is significantly impaired.

The data represent the mean ± s.e.m. for three independent experiments in duplicate for each concentration point. **, P<0.01 versus untreated control.