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. 2011 Nov 1;9:28. doi: 10.1186/1478-811X-9-28

Table 2.

Reported phosphorylation/injection of CagA in human cell linesa

Cell line Origin H. pylori strains used Phospho-CagAb Applied methods References
AGS stomach G27, P1, P12, P210, GU301, GU303, GU304, GU305, GU306, GC401, GC402, 342, ATCC43579, 87A300, NCTC11916, NCTC11637 +++ IP, Anti-PY WB, CF, IF, 2-DE, MS [78,92-95]
MKN-1 stomach P12 + IP, Anti-PY WB [75]
MKN-7 stomach P12 + IP, Anti-PY WB [75]
MKN-28 stomach P1, P12 ++ Anti-PY WB/IPA [74]
MKN-45 stomach P1, P12 +++ Anti-PY WB/IPA [74]
MKN-74 stomach P12 + IP, Anti-PY WB [75]
KATO-3 stomach P1, P12 +++ Anti-PY WB/IPA [74]
23123/87 stomach P12 ++ IP, Anti-PY WB [75]
Hs-746T stomach P12 ++ IP, Anti-PY WB [75]
FU-97 stomach P12 + IP, Anti-PY WB [75]
NUGC-4 stomach P12 + IP, Anti-PY WB [75]
SNU-1 stomach P12 + IP, Anti-PY WB [75]
SNU-5 stomach P12 ++ IP, Anti-PY WB [75]
SNU-16 stomach P12 + IP, Anti-PY WB [75]
HeLa cervix P1, P12 ++ Anti-PY WB/IPA [74,84]
HT-29 colon P1, P12 ++ Anti-PY WB/IPA [74]
Hec1.b endometrium P1, P12 + Anti-PY WB/IPA [74]
293T kidney P1, P12 ++ Anti-PY WB/IPA [74]
HL lung P1, P12 ++ Anti-PY WB/IPA [74]
HepG2 liver P1, P12 ++ Anti-PY WB/IPA [74]
MCF-7 breast P12 +++ Anti-PY WB [72]
THP1 blood P1, P12, ATCC43526 +++ Anti-PY WB [96]
U937 blood P1, P12, G27 +++ Anti-PY WB [97]
Josk-M blood P1, P12, G27 +++ Anti-PY WB [97]
B cells, primary blood HM-6, HM-9 +++ IP, Anti-PY WB [98]
BJAB blood HM-6, HM-9 +++ IP, Anti-PY WB [98]

a Abbreviations: 2-DE (two-dimensional protein gel electrophoresis); anti-PY WB (Westernblotting of infected cell lysates using several pan-phospho-tyrosine antibodies); CF (cell fractionation); IF (localization by immunofluorescence); IP (immunoprecipitation); IPA (in vitro phosphorylation assays of CagA with cell lysates to ensure that active kinases are present in the cells of interest); MS (mass spectrometry of precipitated phospho-CagA).

b Intensity of CagA's tyrosine phosphorylation signals: +++ strong phosphorylation; ++ moderate phosphorylation; + weak phosphorylation.