Figure 5.
PMNs synergize with T cells to enhance in cytokine production without an affect on proliferation. A-P and R. Co-culturing of WT CD4+ T cells with PMNs isolated from colonic LP of colitic mice results in increased production of cytokines and chemokines WT CD4+ T cells were cultured along (cross-hatched bars) or with the indicated number (shown on x-axis) of FACS-sorted PMNs (grey bars) in the presence of activating anti-CD3/CD28 antibodies for 48 hrs. Cytokine concentrations were measured using multiplex cytokine analysis. Data are expressed as the mean±SEM of serial dilutions (undiluted, 1:2 and 1:4) of each sample. Shown are results from a representative experiment of two independently conducted co-culture experiments with similar results. Proliferation of WT T cells in response to anti-CD3/CD28 antibodies in the absence or presence of PMNs (Q) was not substantially increased with the addition of PMNs.