Figure 2.
Enhanced accumulation of myeloid cells in mice with chronic colitis. A. Absolute numbers of CD4+ T cells, Mac-1+Gr-1+ and Mac-1+Gr-1− were quantified in CD45RBhigh→RAG−/− (black bars), CD45RBlow→RAG−/− (white bars) and CD45RBhigh + CD45RBlow→RAG−/− mice (hatched bars). At least 5 animals were analyzed per group and experiments were repeated at least twice. Data are expressed as mean±SEM. * indicates p<0.05, ** indicates p<0.01 and *** indicates p<0.001 compared to the RBhigh control group. B. Representative dot plots showing percentages of Mac-1+Gr-1+ and Mac-1+Gr-1− cells in the spleens, MLNs and colon LP. Numbers indicate percentages of cells within the gated leukocyte population (using forward and side scatter).
